Fig. 3
From: Attenuation of diet-induced hypothalamic inflammation following bariatric surgery in female mice
RYGB and BD-IL procedures normalize HFD-induced hypothalamic microgliosis. a Representative images of GFP fluorescence from bariatric surgical mice and controls taken at Bregma − 1.6 to − 1.9. Top left image shows DAPI staining of mediolbasal hypothalamus at this level, with the dashed lines defining the anatomical borders of the arcuate nucleus (ARC). 3 V, third ventricle. ME, median eminence. Scale bar = 50 μm. b Quantification of microglia in the arcuate nucleus following sacrifice (n = 4–5 mice per group, 2–3 sections per mouse). c Quantification of microglia in other mediobasal hypothalamic nuclei as well as cortex. a = significant difference from DIO-SHAM. p < 0.05 (n = 5–6 per group). Data are represented as mean ± SEM