Table 1 Primer sequences of the germ layer differentiation markers for qPCR assay
Primer sequence (5’—3’) | Primer name | Purification method | Attribution of differentiation marker |
|---|---|---|---|
CATGGCCTTCCGTGTTCCTA | mGAPDH_F | PAG | Internal reference |
CCTGCTTCACCACCTTCTTGAT | mGAPDH_R | PAG | Internal reference |
ACCTGAGCTATAAGCAGGTTAAGAC | mNanog_F | PAG | Sternness |
GTGCTGAGCCCTTCTGAATCAGAC | mNanog_R | PAG | Sternness |
TTACTTCCCGCCAGCTCTTC | rnSoxl_F | PAG | Neuroectederm |
TGATGCATTTTGGGGGTATCTCTC | mSoxl_R | PAG | Neuroectoderm |
GGCAACTATGTAGGGGACTCAG | mTubb3_F | PAG | Neuroectederm |
CCTGGGCACATACTTGTGAG | mTubb3_R | PAG | Neuroectederm |
CTCCAACCTATGCGGACAAT | mT_F | PAG | Mesendoderm |
AGACTGGGATACTGGCTAGAG | mT_R | PAG | Mesendodetm |
CCCATCCTGGACCGTTTCC | mLhxl_F | PAG | Mesoderm |
CGCTTGGAGAGATGCCCTG | mLtaxl_R | PAG | Mesoderm |
CACATGAAGGAGTACCCGGACTA | mSox3_F | PAG | Ncurocctodcrm |
TGAGCAGCGTCTTGGTCTTG | mSox3_R | PAG | Neuroectederm |
CGAGCCAAAGCGGAGTCTC | mSoxl7_F | PAG | Fmlodenri |
TGCCAAGGTCAACGCCTTC | mSoxl7_R | PAG | Fndodcrm |