Fig. 9

Exacerbation of the DNA damage expression in lung tissue from mice treated with bleomycin and vitamin D. a Representative micrograph of a bronchiole surrounded by a fibrotic focus. Two magnifications of the image are depicted in the rectangles on the right. Arrowheads signal some of the multiple nuclei harboring DD foci containing γH2AFX indicative of severe DNA damage. b Representative micrographs of interstitial fibrosis (left) and of an area abnormally re-epithelialized (right) showing the expression of massive DNA damage specially in the epithelial component. c Representative image of a subpleural fibrotic lesion lined by an abnormally re-epithelialized area showing the presence of severe DNA damage especially in epithelial cells. Scale bars: 100 μm