Fig. 2

a Expression of Angpt1 mRNA 1, 3, 7, and 14 days after IRI in wild mice. The expression of Angpt1 increased 7 days and 14 days after IRI. (N = 6 per sham and IRI groups at each time point) b The expression of Angpt1 in kidney was significantly suppressed in the Angpt1 knockout mice on the day of IRI and 1, 3, or 7 days after IRI compared to that in the control mice (N = 6, 6, 12, and 17 in knockout group on day 0, 1, 3, and 7 days respectively, N = 6, 6, 12, and 15 in control group on day 0, 1, 3, and 7 days respectively, P < 0.05 or P < 0.01) c & d Western blotting of mouse Angpt1 3 days after IRI. The Angpt1 protein level was lower in the knockout than control mice. (N = 5 for each group, P < 0.05) e Serum creatinine level was higher in the Angpt1 knockout mice 3 and 7 days after IRI. (N = 17 in knockout group, N = 15 in control group, N = 5 in the sham group, *P < 0.05, **P < 0.01) f Serum BUN level was higher in Angpt1 knockout mice 3 days after IRI. (N = 17 in knockout group, N = 15 in control group, N = 5 in the sham group, *P < 0.05) g PAS staining of renal tissue in control, knockout and sham mice. The remaining tubular necrosis was more severe and the renal tubular recovery was less in the knockout mice 7 days after IRI. Arrow indicates recovery tubule. Arrow head indicates necrotic tubule. h Semi-quantitative analysis of the regeneration score in the control and knockout mice. The recovery percentage was lower and the necrosis percentage was higher in the Angpt1 knockout mice 7 days after IRI (N = 17 in the knockout group, N = 15 in the control group)