Fig. 1
From: LncRNA PVT1 regulates atrial fibrosis via miR-128-3p-SP1-TGF-β1-Smad axis in atrial fibrillation
PVT1 is increased in AF patients and positively with collagen I and collagen II. The atrial muscle tissues were collected from SR (n = 20) and AF patients (n = 30) and subjected to the following experiments. a qRT-PCR analysis of PVT1 expression in human atrial muscle tissues. b qRT-PCR analysis of PVT1 expression from AF patients with different cardiac function classification (NYHA I-IV). PVT1 expression was gradually elevated with the increase of cardiac function classification. c qRT-PCR analysis of Collagen I and Collagen III mRNA levels in human atrial muscle tissues. d Western blot analysis of Collagen I and Collagen III protein levels in human atrial muscle tissues. β-actin served as the loading control. e Immunohistochemistry analysis of Collagen I showing the Collagen I-positive signal (brownish-yellow granules). Scale bar: 25 μm. f The positive correlation between PVT1 and Collagen I/III expression in human atrial muscle tissues from AF patients. a, c-f *P < 0.05 vs. SR; b *P < 0.05 and **P < 0.01. Data are presented as mean ± SD. SR, sinus rhythm; AF, atrial fibrillation; PVT1, plasmacytoma variant translocation 1