Table 1 Timeline for each experiment. Abbreviations: FDG, fluorodeoxyglucose; PET, positron emission tomography
DAY | Time | Procedure |
|---|---|---|
1 | 9:00–10:00 | Object Place Memory task #1 |
1 | 10:10–10:55 | FDG injection and uptake |
1 | 10:55–11:05 | Pre-alcohol microPET scan under anesthesia |
1–2 | 11:05–16:00 | Tracer decay for 29–30 h |
2 | 16:00–18:00 | Vein cannulation, alcohol bolus (i.v.) under anesthesia |
2 | 18:00–19:00 | Recovery |
2–3 | 19:00–9:00 | Alcohol infusion via pump, without anesthesia |
3 | 9:00–9:10 | Vein ligation after 15 h under light anesthesia |
3 | 9:20–10:05 | FDG injection |
3 | 9:20–10:05 | Object Place Memory task #2 (assessed during FDG uptake) |
3 | 10:05–10:15 | Post-alcohol microPET scan under anesthesia |
3 | 10:20 | Euthanasia |