Fig. 4

Poly I:C treatment increased the expression of circRNA-9119. a Pentobarbital sodium (50 mg/kg) was used to anaesthetize mice. Testes were revealed via operation. Testes in the experimental group received 0.3 μg of poly I:C, while those from the control group were injected with PBS. At various time points, testes were homogenized and examined for circRNA-9119 concentration with qRT-PCR. b Sertoli cells, Leydig cells, and male germ cells were separated from C57BL/6 mice after 6 h treatment with poly I:C or PBS. Total RNAs were isolated from cells, while circRNA-9119 concentration was evaluated with qRT-PCR after normalization to GAPDH level. Cells without poly I:C treatment served as controls (Ctrl). Results are shown as means ± SEM (n = 3 replicates each experiment). *P < 0.05, **P < 0.01, ***P < 0.001