Fig. 2

mTAT is the most efficient CPP in transferring R16/17.GFP.Pal protein to the muscle of ΔR4 mice. a Transduction efficiency of five different CPPs, including TAT, mTAT, R10, FHV and ANTP, in delivering R16/17.GFP.Pal protein to the muscle of ΔR4 mice was compared. R16/17.GFP.Pal protein carrying different CPPs was injected to ΔR4 mice, and the distribution of R16/17.GFP.Pal protein in the TA muscle was evaluated by direct fluorescence microscopy for GFP signal, and immunofluorescence staining for GFP and R17 on serial sections of the TA muscle. As the images shown here, mTAT is the most efficient CPP in transferring R16/17.GFP.Pal protein to TA muscles of ΔR4 mice. Asterisk: the same myofiber on serial sections. Scale bar = 50 μm; b The intensity of the signal of GFP and R17 immunostaining was quantified and confirmed that mTAT led to the highest distribution of R16/17 protein in the muscle