Fig. 6

NF-κB is involved in YAP regulated transcription of IL-11 in EC cells. (a) Ishikawa or AN3CA cells were treated with or without inhibitor of AP-1 (SR 11302, 10 μM), SMAD2/3 (SB431542, 10 μM), and NF-κB (BAY 11–7082, 10 μM), respectively, for 24 h, the mRNA of IL-11 was measured by use of qRT-PCR; (b) Cells were transfected with vector control or pcDNA/p65 for 24 h, the expression of p65 was measured by western blot analysis; Ishikawa (c) or AN3CA (d) cells were pre-transfected with si-NC or si-YAP for 6 h, and then further transfected with vector control or pcDNA/p65 for 24 h; (e) Cells were treated with VP for the indicated times, the mRNA of p65 was measured by use of qRT-PCR. Data are presented as means ± SD of three independent experiments. ** p < 0.01 compared with control