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Fig. 4 | Molecular Medicine

Fig. 4

From: High co-expression of TNF-α and CARDS toxin is a good predictor for refractory Mycoplasma pneumoniae pneumonia

Fig. 4

Plasmid construction and vacuolation activity verification of CARDS toxin. a Schematic diagram of plasmid construction. CARDS toxin gene (MPN372) eukaryotic expression vector was constructed and purified. b Purity of CARDS toxin by SDS-PAGE. c HeLa cells were co-cultured with 50 μg/mL CARDS toxin or not for 24 h. CARDS toxin exhibited the ability to induce vacuolization in HeLa cells compared with the control group.

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