Fig. 4

PX-866 blocks autophagy in glioma cells. a T98G cells were treated with increasing doses of PX-866 for 24 h and then total protein was isolated. b T98G cells were subjected to single treatment with 3-MA (2.5 mM) or co-treatment with either PX-866 (1 μM) or Baf (10 nM) for 24 h, according to the treatment legend. c The LC3 flux assay was performed on T98G cells at 4 h and 24 h using Baf (10 nM), PX-866 (1 μM) or a combination of the two. Data represents three independent experiments