Fig. 6
Immunoblot analysis of T-tubule and SR proteins. a Representative immunoblots of microsomal fractions from LV tissues of each study group that were probed with anti-BIN1, Jph2, Cav1.2 and RyR2 antibodies. Observed molecular weights (kDa) of each protein are indicated. Ponceau red staining of the immunoblots are shown with molecular weight markers. b,c Bar graphs show the quantitation of the luminescence intensity of the indicated protein bands, normalized to Ponceau red staining and expressed relative to the mean sham values. Values are mean ± SD for n = 4–6 hearts/group. Statistical analysis using one-way ANOVA with post-hoc Tukey’s multiple group comparisons; p-values are indicated