Fig. 4
From: Down-regulated miR-495 can target programmed cell death 10 in ankylosing spondylitis
Validation the interaction between PDCD10 and miR-495. a Schema of the interaction between miR-495 and PDCD10 3′UTR. Black vertical lines and red bases indicate the interacting regions. Mutated bases are marked with green letters. b Dual luciferase report assay results. Vector PDCD10 and VectorMut-PDCD10 indicate plasmid with wild-type and mutated PDCD10 3′UTR, respectively. MiR-495 PDCD10 indicates the interaction between wild-type miR-495 and wild-type PDCD10 3′UTR. MiR-495 Mut-PDCD10 indicates the interaction between wide type miR-495 and mutated PDCD10 3′UTR. Mut-miR-495 PDCD10 indicates the interaction between mutated miR-495 and wild-type PDCD10 3′UTR. Mut-miR-495 Mut-PDCD10 indicates the interaction between mutated miR-495 and mutated PDCD10 3′UTR. c Transfection of primary PBMCs from AS. Top picture: bright field image of transfected PBMCs. Bottom picture: fluorescence image of transfected PBMCs. d Detection of miR-495 in transfected primary PBMCs from AS. Mock: untreated group. Vector: blank vector group. Control: mutated miR-495 over-expression group. MiR-495: miR-495 over-expression group. e RNA detection of PDCD10 in transfected primary PBMCs from AS. Mock: untreated group. Vector: blank vector group. Control: mutated miR-495 over-expression group. MiR-495: miR-495 over-expression group. f Western blot of PDCD10 in transfected primary PBMCs from AS. Mock: untreated group. Vector: blank vector group. Control: mutated miR-495over-expression group. MiR-495: miR-495 over-expression group. n = 6: repeated times. ***: P value < 0.01