Fig. 2

Identification of SP1 as the mutual transcription factor of MRPs and 12LOX. a Bioinformatic analysis of transcription factors of MRP1, MRP4, Snail, Slug, 12LOX, COX1, and CYP2J2. b SP1 binding sequences in the promoters of MRP1 (ABCC1), MRP4 (ABCC4), and 12LOX (ALOX12). c ChIP assay of SP1 binding on the promoters of MRP1 (ABCC1), MRP4 (ABCC4), and 12LOX (ALOX12). d, e The mRNA (d) and protein (e) expression levels of SP1 in SKOV3 and SKOV3-R cells. Data in (d) are expressed as mean ± SEM of 4 independent experiments. ^***P < 0.001. f The unpaired Student’s t test was used to analyze the expression of SP1 in the GEO database (GSE114206). Data are expressed as mean ± SEM and ^*P < 0.05. g Patient survival data were obtained from TCGA database and overall survival probability was then calculated using the Kaplan-Meier method and the differences in survival curves were analyzed using the log-rank test. P-values of 0.05 or less were considered significant