Fig. 1
From: iNOS/NO is required for IRF1 activation in response to liver ischemia-reperfusion in mice
PUMA induction is dependent on iNOS wild-type in response to I/R. a Mouse liver I/R was performed with 1 h ischemia and 6 h reperfusion in C57BL/6 mice (n = 4). iNOS expression in the liver tissues was analyzed by Western blot. b Similar to (a), but 6, 12, 24 and 48 h reperfusion I/R were performed in iNOS+/+ and iNOS−/− mice. RT-PCR detected mRNA coding for iNOS gene. c Liver tissues were collected from I/R iNOS+/+ and iNOS−/− mice with ischemia for 1 h and reperfusion for the indicated times. qRT-PCR was carried out with primers for PUMA gene and normalized to β-actin in iNOS+/+ compared with iNOS−/− mice at each time point, P < 0.0001. Data represent the mean ± the standard deviation (SD), n = 4. d Similar as (c), but PUMA expressions were analyzed in total proteins by Western blot