Fig. 10

Glycolysis enzyme levels are elevated in caspase-1/11KO. a Western blot images of expression of Protein Kinase M (PKM), enolase, aldolase, Protein Kinase L/R (PKLR), TIM30 and actin loading control in muscle stellate cells (MuSC) at 5 days after harvest from tibialis anterior (TA) nonischemic and ischemic muscle at 21 days after femoral artery ligation (FAL) in C57Bl/6 J (WT) and caspase-1/11KO mice treated with PBS or CQ (50 mg/kg) (b-f) Quantification of relative expression of each enzyme relative to actin using Image J gel analyzer was used to quantify relative expression to that of actin loading control; (b) PKM *p < 0.03 PBS:CQ WT and CQ in WT:KO, **p < 0.001 PBS in WT:KO and PBS:CQ KO; (c) enolase *p < 0.03 PBS: CQ in WT and KO and CQ in WT:KO; (d) PKLR **p < 0.001 PBS:CQ in KO and PBS in WT:KO; (e) aldolase *p < 0.03 PBS:CQ in KO, **p < 0.001 PBS in WT:CQ; (f) TIM30; (g) oxygen consumption rate (OCR) and (h) extracellular acidification rate (ECAR) measured by Seahorse analyzer in MuSC from WT and caspase-1/11KO mice, N = 3 mice per strain per treatment