Fig. 3
From: THRIL mediates endothelial progenitor cells autophagy via AKT pathway and FUS
THRIL inhibits proliferation and promoted apoptosis through binding with FUS (a left) The expression of THRIL in CAD EPCs cell nucleus and cytoplasm was measured through northern blotting. (a right) The expression of FUS in CAD EPCs cell nucleus and cytoplasm was measured through western blotting. (b-d) The direct interactions between THRIL and FUS was confirmed through RNA electrophoretic mobility shift assays (RNA EMSA) and RNA immunoprecipitation (RIP) assays. (e) The effects of THRIL over-expression on cell viability of CAD EPCs was reversed through knockdown of FUS. (f) The effects of over-expression of THRIL on cell apoptosis of CAD EPCs was reversed through knockdown of FUS. NC: EPCs isolated from CAD patients; All the transfection experiments were performed in EPCs isolated from CAD patients. Data are presented as mean ± SD. *P < 0.05. The data shown represent three separate experiments performed in triplicate in each experiment (mean ± the standard deviation [SD])