Fig. 4From: Fetal circulating human resistin increases in diabetes during pregnancy and impairs placental mitochondrial biogenesisEffect of resistin on mitochondrial biogenesis in placentae. Human placental explants were treated with indicated doses of human resistin for 24 h. a protein lysates were subjected for Western blot analysis; b total DNA were extracted and mitochondrial DNA copy number was determined by fold change of mitochondrial tRNALeu(UUR) gene DNA copy number normalized to nuclear β2 microglobin (B2M) with quantitative RT-PCR. Mean ± SD, ** P < 0.01; *** P < 0.001; N = 4. # P < 0.05 with one-tail T-testBack to article page