Fig. 5

LincRNA PADNA/miR-194/FBXW7 axis in bupivacaine-induced neurotoxicity. a Knockdown of lincRNA PADNA significantly reduced the expression of FBXW7 mRNA in DRG neurons. b Knockdown of lincRNA PADNA significantly reduced the expression of FBXW7 protein in DRG neurons. c, d Knockdown of miR-194 reversed the expression of FBXW7 in DRG neurons which is inhibited by knockdown of lincRNA PADNA. e Knockdown of miR-194 reversed the up-regulated percentage of apoptotic of DRG neurons which is induced by the knockdown of lincRNA PADNA. Cells were treated with 1 mM bupivacaine. f Downregulated of miR-194 reversed the up-regulated caspase3 activity of DRG neurons which is induced by the knockdown of lincRNA PADNA. Cells were treated with 1 mM bupivacaine. g Downregulated of miR-194 reversed the down-regulated cell viability of DRG neurons which is induced by the knockdown of lincRNA PADNA. Cells were treated with 1 mM bupivacaine. h Downregulated of miR-194 reversed the up-regulated expression of caspase3 protein in DRG neurons which is induced by the knockdown of lincRNA PADNA. Cells were treated with 1 mM bupivacaine. Data are Mean ± SD with n = 3 independent biological cultures. *p < 0.05 and ** p < 0.01