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Fig. 4 | Molecular Medicine

Fig. 4

From: Long noncoding RNA HCG18 inhibits the differentiation of human bone marrow-derived mesenchymal stem cells in osteoporosis by targeting miR-30a-5p/NOTCH1 axis

Fig. 4

Effect of miR-30a-5p and NOTCH1 on osteogenic differentiation of BMSCs. a Putative binding sites for NOTCH1 and miR-30a-5p. b Luciferase activity of NOTCH1-wt and NOTCH1-mut in miR-30a-5p mimics or NC-treated BMSC cells. c Effect of overexpression of miR-30a-5p on the expression of NOTCH1. d Effect of overexpression of miR-30a-5p on the expression of NOTCH1 protein. e Effect of overexpression of HCG18 on the expression of NOTCH1. f Effect of overexpression of HCG18 on the expression of NOTCH1. g Effect of HCG18 knockdown on NOTCH1 mRNA expression. h Effect of HCG18 knockdown on NOTCH1 protein expression. i ALP activity assay. j Determination of the expression of osteogenic differentiation specific markers (Runx2, OCN, OPN). * P < 0.05. The OP-BMSCs were from one human subject and the experiments were performed in three replicates

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Molecular Medicine

ISSN: 1528-3658