Fig. 2

Numerous cellular processes were affected by KBTBD8, as assessed by RNA sequencing. a, b Blot and quantification showed that KBTBD8 protein level was about sixfold higher in A2780 EOC cells than in normal epithelial ovarian cell line moody (KBTBD8 protein level, moody vs. A2780, 0.04061 vs. 0.2623). c, d Blot and quantification showed that KBTBD8 protein level increased in KBTBD8-knockdown-overexpressed (K8-KD-OE) and KBTBD8-overexpressed (K8-OE) A2780 cells and decreased in KBTBD8-knockdown (K8-KD) A2780 cells compared with the control A2780 cells (CTR vs. K8-KD vs. K8-KD-OE vs. K8-OE, 1.259 vs. 0.4056 vs. 1.692 vs. 2.137). e Scattered map of upregulated (red) and downregulated (blue) genes which were positioned away from unchanged genes (gray). f Pie Chart of differentially expressed genes. 146 genes were significantly upregulated (|log2FC|≥ 1), and 448 genes were significantly downregulated (|log2FC|≥ 1). The downregulated genes cover 76.97% (448/594) of all differentially-expressed genes. g Heat map of differentially-expressed genes between CTR and KBTBD8-knockdown group. h–j Q-PCR of some screened genes verified the creditability of the sequencing, and K8-OE could rescue the changes caused by K8-KD. k KEGG analysis showed that many of the proteins that these genes encode are involved in multiple tumorigenesis pathways. l Protein interaction analysis by String showed that that 39 functionally essential proteins encoded by differentially expressed genes interact with multiple kinases that are known to be important for tumorigenesis. Different characters above the column between groups indicate significant difference. ***Indicates p < 0.001, ****indicates p < 0.0001