Downregulation of the ubiquitin ligase KBTBD8 prevented epithelial ovarian cancer progression

Table 1 Correlation of KBTBD8 protein level and the malignance grade of the ovarian tissues

Feature	KBTBD8 staining score^a			N^b	p value^c
Feature	−	+	++	N^b	p value^c
Age(years)
≤ 48	8	16	11	35	0.037
> 48	3	17	15	35
TNM-stage
–	4	2	6	12	0.03
TI	6	15	11	32
TII	0	10	5	15
TIII	1	6	4	11
Lymph node metasitasis
Yes	0	6	3	9	0.688
No	11	27	23	61
Multifocality
Yes	0	2	0	2	0.626
No	11	31	26	68
Disease grade
–	9	4	5	18	0.00
1	1	8	5	14
2	1	11	3	15
3	0	10	13	23
TNM stage group
–	4	2	6	12	0.021
I	6	15	11	32
II	0	8	5	13
III	1	6	4	11
IV	0	2	0	2
Percent of positive cells(PP)
≤ 50	11	23	2	36	0.00
> 50	0	10	24	34

This table was generalized from Additional file 1: Dataset 1. The intensity score of KBTBD8 staining of each array point was standardized as follows: 0, no staining; 1, weak staining; 2, moderate staining; and 3, strong staining. For each array point, at least 500 randomly-picked cells from five random areas were observed and analyzed by two independent professional analyzers to generate a final average score
The proportion score of KBTBD8-stained tumor cells was standardized as follows: 0, < 5% of cells with positive KBTBD8 staining; 1, 5–25%; 2, 25–50%; 3, 50–75%; and 4, > 75%
Next the final score was obtained by the intensity score added together with the proportion score. A final score of 0–1 was defined as (−), while scores of 1–2, 3–4, and 5–6 were considered (+), (++) and (+++), respectively
^aThe KBTBD8 antibody staining Score method is detailed as follow
^bN stands for the number of cases
^cp values were calculated by Kruskal–Wallis H test tests. A value of p < 0.05 was considered statistically significant

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