Fig. 3
From: LncRNA KCNA2-AS regulates spinal astrocyte activation through STAT3 to affect postherpetic neuralgia
The suppression of KCNA2-AS inhibits the activation of NO to astrocytes. a As described previously, the correlation between NO content and GFAP expression in spinal cord tissues previously tested in vivo was analyzed. b Primary rat spinal astrocytes were treated with s-nitrosylglutathione (GSNO, NO donor) for different time periods (0, 2, 6, 12, and 24 h). The GFAP and KCNA2-AS expression were detected by qRT-PCR. c, d Primary rat spinal astrocytes were transfected with or without si-KCNA2-AS, and treated with GSNO for 24 h. The control group was untreated cells. The GFAP expression was detected by qRT-PCR (c). Immunofluorescence staining was performed by using GFAP antibody and DAPI. GFAP, red staining; DAPI / nucleus, blue staining (d). **P < 0.01 vs. 0 h group or control group; #P < 0.05 and ##P < 0.01 vs. GSNO group