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Fig. 3 | Molecular Medicine

Fig. 3

From: Downregulation of long noncoding RNA DLEU1 attenuates hypersensitivity in chronic constriction injury-induced neuropathic pain in rats by targeting miR-133a-3p/SRPK1 axis

Fig. 3

DLEU1 bound with miR-133a-3p. a FISH assay was conducted to detect the DLEU1 subcellular localization in rat microglial cells. b RT-qPCR analysis was conducted to detect the relative expression of miRNAs in rat microglial cells by indicated transfections. c MiR-133a-3p expression in rat microglial cells was overexpressed by transfection with miR-133a-3p mimics and the efficiency of transfection was determined by RT-qPCR analysis. d Luciferase reporter assay was conducted to confirm the relationship between miR-133a-3p and DLEU1 in HEK-293T cells. e RT-qPCR analysis was conducted to detect the expression of DLEU1 in rat microglial cells transfected with miR-133a-3p mimics. f RT-qPCR analysis was conducted to assess the expression of miR-133a-3p in spinal cord tissues after CCI surgery for 0, 7, 14, 21 days. *p < 0.05, **p < 0.01

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Molecular Medicine

ISSN: 1528-3658