Fig. 4

Acupuncture repressed cerebral ischemia–reperfusion (I/R) injury-induced endoplasmic reticulum (ER) stress via inhibiting transcription factor 6 (ATF-6) expression. Immunofluorescence analysis of ATF-6 expression (red) in cortex (a–c), hippocampus (d–f), and striatum (g–i) of contralateral and ipsilateral brains at 72 h after reperfusion. The nuclei were counterstained with DAPI (blue). Scale bars, 100 μm. Results represent at least three independent experiments. Each experimental result was presented as mean ± standard deviation (n = 6). ^##P < 0.01, ^###P < 0.001 versus the indicated group