Fig. 3

miR-17 in the miR-17-92 cluster targets EZH1 and downregulates its expression in primary lung fibroblasts of BPD mice. a The binding site of miR-17 and EZH1 predicted by Targetscan website. b The expression of miR-17 in cells transfected with miR-17 mimic and miR-17 inhibitor assayed by RT-qPCR. c The binding of miR-17 to EZH1 assayed by dual-luciferase reporter gene assay. d The expression of EZH1 in cells transfected with miR-17 mimic and miR-17 inhibitor assayed by RT-qPCR and Western blot analysis. e The expression of EZH1 in fibroblasts transfected with sh-Hdac3, or both sh-Hdac3 and miR-17 inhibitor assayed by RT-qPCR and Western blot analysis. *p < 0.05 compared to inhibitor NC mimic NC, or sh-NC. The above data were expressed as mean ± standard deviation. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test