Fig. 4

EZH1 elevates the expression of Pgf via stimulating p65 transcription factor expression in primary lung fibroblasts of BPD mice. a The binding sites between p65 and Pgf. b The expression of EZH1 in cells transfected with oe-EZH1 assayed by RT-qPCR and Western blot analysis. c The binding of EZH1 and p65 assayed by immunoprecipitation assay. d The enrichment of p65 in the Pgf promoter region in oe-EZH1-treated cells assayed by ChIP assay. e The expression of p65 in sh-p65-treated cells assayed by Western blot analysis. f The expression of Pgf in sh-p65-treated cells assayed by RT-qPCR and Western blot analysis. g The expression of Pgf in fibroblasts transfected with oe-EZH1, or both oe-EZH1 and sh-p65 assayed by RT-qPCR and Western blot analysis. *p < 0.05 compared to oe-NC or sh-NC. The above data were expressed as mean ± standard deviation. The data between the two groups with unpaired design were compared by unpaired t-test. Data among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post-hoc test