Table 1 Primer sequences for RT-qPCR
Target | Primer sequence (5′-3′) |
|---|---|
miR-17-92 | F: TGAGGGCACTTGTAGCATTATG |
R: CCTTAGAACAAAAAGCACGCAG | |
miR-17-5p | F: GCGAATTCCAAATTTAGCAGGAATAAAG |
R: CGCTCGAGGACTGGACGCAGCCAGTG | |
Pgf | F: TTCCCCTTGGTTTTCCTCCTT R: AGATCTTGAAGATTCCCCCCA |
GAPDH | F: AAATGGTGAAGGTCGGTGTGAACG |
R: ATCTCCACTTTGCCACTGC | |
U6 | F: GAACCTCACCTTGGGACTGA |
R: TGCTACAAGTGCCCTCACTG |