Fig. 1

Schematic of the generated FINCA allele and body weight curve of Nhlrc2^FINCA/− and Nhlrc2^+/+ mice. a Modified region of mouse and human amino acid (aa) and nucleotide sequence (nt) of the modified region. Silent mutation leading to removal of the PAM site and resulting in the addition of a TatI restriction site (green) was included in the ssODN. FINCA variant c.442G > T (red) and amino acid substitution p.Asp148Tyr (yellow) are shown. b Body weight curve showing no significant difference in weight gain for either sex between Nhlrc2^FINCA/− and Nhlrc2^+/+ mice during the 32- week observation period (two-way ANOVA). Nhlrc2^FINCA/− male (N = 6), Nhlrc2^+/+ male (N = 6) Nhlrc2^FINCA/− female (N = 6) Nhlrc2^+/+ female (N = 5). Mean and standard deviation (SD) shown