Fig. 5

ROCK1 plays a key role in mediating H₂O₂-induced lens epithelial cells apoptosis. a Western blotting confirmation of the siRNA-mediated ROCK1 knockdown. Cells were lysed for western blotting after transfection with si-ROCK1 and si-NC, respectively. β-actin was used as an internal reference. Data are based on three independent experiments. The 100–250 kDa blot was retained by horizontal shear and incubated with the ROCK1 antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody. b. Cells transfected with si-ROCK1 were treated with H₂O₂ for 24 h and cell survival was tested by the MTT assay (n = 5). **p < 0.01 versus untreated group. ^##p < 0.01 versus H₂O₂-treated group. Data are based on three independent experiments. c ROCK1-knockdown suppressed H₂O₂-induced cell apoptosis by apoptotic assay. Cells transfected with si-ROCK1 were treated with H₂O₂ for 24 h, and cell apoptosis was visualized by Hoechst 33342 staining assay. d Cell apoptosis was analyzed after transfection with si-ROCK1 plasmids. **p < 0.01 versus untreated group. ^##p < 0.01 versus H₂O₂-treated group. Scale bar: 50 µm. e Western blotting confirmation of the over-expression levels of ROCK1 after transfection with the pcDNA-ROCK1 plasmid. The control group was transfection with the pcDNA-NC plasmid. The 100–250 kDa blot was retained by horizontal shear and incubated with the ROCK1 antibody. The 35–50 kDa blot was retained by horizontal shear and incubated with the β-actin antibody. f ROCK1 over-expression decreased B3 cell survival was determined by MTT analysis. Cell survival was tested after transfection with pcDAN-ROCK1 plasmids by the MTT assay (n = 5). **p < 0.01 versus untreated group. g ROCK1 over-expression induced cell apoptosis in B3 cells by apoptotic assay. Scale bar: 50 µm. h Cell apoptosis was analyzed after transfection with ROCK1 overexpression plasmids. **p < 0.01 versus the untransfected group