Fig. 1
mRNA microarray analysis and up-regulated expression of G6PD in gastric cancer. a The heat map illustrated the differently expressed mRNAs in gastric tumor and normal tissues based on online datasets GSE51575, GSE63089 and GSE81948. b G6PD was both highly expressed in gastric cancer tissue samples of GSE51575, GSE63089 and GSE81948 datasets. c The expression levels of G6PD in gastric cancer tissue samples and corresponding adjacent normal tissue samples, based on online datasets GSE29272 and GSE63089. d The mRNA expression of G6PD was examined in 77 paired gastric cancer and adjacent non-cancerous tissues by qRT-PCR. e The protein levels of G6PD were examined in tissue samples by western blot. f The mRNA expression of G6PD was detected in human gastric epithelial cell line (GES-1) and gastric cancer cell lines (AGS, MGC-803, SGC-7901 and MKN-45 cells) by qRT-PCR. g The protein levels of G6PD was examined i human gastric epithelial cell line and gastric cancer cell line by western blot. *P < 0.05, **P < 0.01 compared to GES-1 group. h Kaplan–Meier survival analysis confirmed that the low expression of G6PD was related to the better prognosis of gastric cancer patients (log-rank test, P < 0.01)