Fig. 3

Knockdown of G6PD inhibited tumor formation in nude mice. a Subcutaneous xenotransplant tumor model was established in nude mice by injecting AGS cells infected with lentivirus-mediated shRNA targeting G6PD (sh-G6PD#1 and sh-G6PD#2) or negative control. The G6PD mRNA expression level in tumor tissues was examined by real-time qPCR. b Tumor volume was measured every three days from the tenth day after injection, when the tumor began to form. c On the 25th day, nude mice were sacrificed and the tumor weight was measured. d Images of the tumors in each group. e The histopathological characteristics of the tumors were examined by hematoxylin and eosin (H&E) staining (100 ×). f The protein content and distribution of G6PD in tissue samples were examined using Immunohistochemical (IHC) staining (× 200). N = 6; **P < 0.01, compared with Lv-sh-NC group