Fig. 6

The regulatory network of LINC00242/ miR-1-3p/ G6PD axis. a Six lncRNAs (LINC00707, MALAT1, NEAT1, SNHG14, UCA1 and LINC00242) targeted miR-1-3p was screened according LncBase Predicted v.2 database and ENCORI database. b RT-PCR assay was performed to detect the expression level of these six lncRNAs in gastric cancer tissues and paracancerous tissues. N = 77; **P < 0.01 compared to adjacent tissues. c LINC00242 expression level in gastric cancer cell lines (AGS and MGC-803) and gastric epithelial cell line (GES-1) was detected. N = 3; *P < 0.01 compared to GES-1 cell. d Kaplan–Meier survival analysis confirmed that the low expression of LINC00242 was related to the better prognosis of gastric cancer patients (log-rank test, P < 0.05). e Target relationship between LINC00242 and miR-1-3p was predicted by bioinformatics analysis (left) and validated by dual-luciferase reporter assay (right). N = 3; **P < 0.01 compared to mimics NC group; ^##P < 0.01 compared to inhibitor NC group. f The linear analysis revealed a negative correlation between LINC00242 and miR-1-3p expression in gastric cancer tissues (N = 77). g The linear analysis revealed a positive correlation between LINC00242 and G6PD expression in gastric cancer tissues (N = 77). h The effect of knockdown of LINC00242 on miR-1-3p expression in AGS and MGC-803 cells was verified by qRT-PCR. N = 3; **P < 0.01 compared to sh-NC group. i, j The effect of silencing of LINC00242 on G6PD mRNA and protein expression in AGS and MGC-803 cells was verified by qRT-PCR and western blot assay. N = 3; **P < 0.01 compared to sh-NC group