Fig. 7

Effects of LINC00242 knockdown on glycolysis and growth of gastric cancer cells in vitro and in vivo. a AGS and MGC-803 cells were separated into six groups: sh-NC + inhibitor NC group (cells transfected with empty plasmid), sh-LINC00242#1 group (cells transfected with shRNA-LINC00242#1), sh-LINC00242#2 group (cells transfected with shRNA-LINC00242#2), miR-1-3p inhibitor group (cells transfected with miR-1-3p inhibitor), Mix1 group (cells transfected with shRNA-LINC00242#1 + miR-1-3p inhibitor) and Mix2 group (cells transfected with shRNA-LINC00242#2 + miR-1-3p inhibitor).. The LINC00242 mRNA expression level was detected in four experiment groups by qRT-PCR. b The miR-1-3p mRNA expression level was detected in four experiment groups by qRT-PCR. c Cell viability was detected by MTT assay. d Cell apoptosis was determined by Flow Cytometry assay. e–g Glucose consumption (e), lactate production (f) and ATP production (g) were determined in four experiment groups. N = 3; **P < 0.01 compared to sh-NC + inhibitor NC group. h AGS cells pre-transfected with lentivirus-mediated shRNA-LINC00242#1, shRNA-LINC00242#2 or shRNA-control were subcutaneous injected into the back of nude mice respectively. The transfection efficiency of LINC00242 was validated by qRT-PCR. i–k The effect of LINC00242 silencing on tumor volume (i), weight (j) and growth (k) were verified. N = 6; **P < 0.01 compared to lv-sh-NC group