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Fig. 1 | Molecular Medicine

Fig. 1

From: Exosomal miR-335 derived from mature dendritic cells enhanced mesenchymal stem cell-mediated bone regeneration of bone defects in athymic rats

Fig. 1

Identification of exosomes and BM-MSCs. The exosomes isolated from imDCs and mDCs were referred to as imDCs-Exo and mDCs-Exo, respectively. a The morphological characterization of imDCs-Exo and mDCs-Exo by TEM. b The particle size of imDCs-Exo and mDCs-Exo measured using nanoparticle tracking analysis. c Expression of the exosomal markers (CD63, and Alix) and the exosome negative marker (Calnexin) confirmed by western blot. d The surface antigens including CD29, CD44, CD90, CD34, and CD45 of rat BM-MSCs was detected by flow cytometry. The positive rate of each surface marker was presented. e Osteogenic differentiation of BM-MSCs was confirmed by Alizarin Red staining. f Adipogenic differentiation of BM-MSCs was identified using Oil Red O staining

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Molecular Medicine

ISSN: 1528-3658