Fig. 3

mDC-Exo promoted osteogenic differentiation of BM-MSCs by transferring miR-335. a Higher expression of miR-672, miR-335, miR-124, and miR-125a-5p in mouse mDC-Exo relative to imDC-Exo (GSE33179). *P < 0.05, vs. imDC-Exo. b Expression of miR-672, miR-335, miR-124, and miR-125a-5p determined by qRT-PCR analysis in imDC-Exo and mDC-Exo separated according to the method mentioned above. *P < 0.05, vs. imDC-Exo. c miR-335 expression determined by qRT-PCR analysis in mDC-Exo-NC and mDC-Exo-miR-335I. **P < 0.01, vs. mDC-Exo-NC. d ALP activities expressed in optical density (OD) values at 520 nm, e, f Runx2 mRNA and protein levels, and g miR-335 expression in BM-MSCs in the groups of control, induction, mDC-Exo, and mDC-Exo-NC, and mDC-Exo-miR-335I on the 7th day of culture. *P < 0.05, **P < 0.01, vs. Control; ^#P < 0.05, ^##P < 0.01, vs. mDC-Exo-NC. h The exosomes isolated from Cy3 (red)-miR-335-transfected mDCs were labeled with the lipophilic fluorescent dye Dio (green) and incubated with BM-MSCs (nuclei stained with Hoechst 33,342, blue). The uptake of exosomes by BM-MSCs was observed by confocal laser microscopy. (I) ALP activities expressed in OD values at 520 nm, (J-K) Runx2 mRNA and protein levels in BM-MSCs transfected with miR-335 mimics and mimic NC. **P < 0.01, vs. mimic NC