Fig. 1

Low-dose MaR1 prevents liver I/R injury. WT mice were injected via tail vein with either PBS (control) or MaR1 at doses of 5 ng, 10 ng or 20 ng/mouse at the beginning of reperfusion after 1 h ischemia. a Graphical scheme of the experimental design. b Serum MaR1 levels of WT mice subjected to hepatic I/R for 6 h or a sham procedure (n = 4 for sham group; n = 6 for I/R group). Serum ALT (c) and AST (d) at 6 h post-reperfusion. Plots show levels in individual mice (n = 6/gp) with bars showing mean ± SEM. Liver H&E (original magnification ×20) from WT mice given PBS (control) or MaR1 (20 ng/mouse) after 1 h ischemia followed by 6 h reperfusion or sham procedure (e). (f) Suzuki’s histological score of liver damage (n = 5/gp); g Dotted lines indicate measured necrotic areas (quantified in bar graph (n = 5/gp); Images are representative across all measured samples. Data are presented as mean ± SEM. *P\(<\)0.05, **P\(<\)0.01