Fig. 4

Expression of LC3-2 and ATG 7 in STZ-induced DN. WT and IL-17A KO mice (n = 6/group) were injected with STZ (50 mg/mL) for 5 consecutive days (citrate buffer was administered to control mice). The animals were euthanized 12 weeks after STZ injection. a Representative images of immunohistochemically stained of LC3-2 (upper) and ATG 7 (lower) of paraffin-embedded kidney sections. At 400× magnification. Quantification results for immunohistochemistry of LC3-2 (b) and ATG7 (c). The mice were randomly assigned to four groups of six mice: (i) wild-type mice without STZ treatment (WT Cont group), (ii) STZ-treated WT diabetic mice (WT STZ group), (iii) IL-17A knockout (KO) mice without STZ treatment (IL-17A KO Cont group), and (iv) STZ-treated IL-17A KO diabetic mice (IL-17A KO STZ group)