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Table 3 RT-LAMP detection of 18S gene used as internal control

From: Essential properties and pitfalls of colorimetric Reverse Transcription Loop-mediated Isothermal Amplification as a point-of-care test for SARS-CoV-2 diagnosis

 

CT RNAse P

CT < 30

CT > 30

RTq-PCR

 Positive

94.8%

37.5%

n = 109

n = 15

 Negative

1.7%

57.5%

n = 2

n = 23

RT-LAMP 18S

 Intermediate

3.5%

5.0%

n = 4

n = 2

 Total

74.2%

25.8%

n = 115

n = 40

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Molecular Medicine

ISSN: 1528-3658