Fig. 3

miR-34a sensitizes to TMZ in a wide range of glioblastoma cultures. a Glioblastoma cultures were reverse-transfected with miR-34a or control miRNA. Two days later, these cultures were treated with TMZ for 4 days. Dashed lines represent mean SRB values (± SD) of TMZ response in control transfected cells and solid lines represent mean SRB values (± SD) of TMZ response miR-34a transfected cells from three independent experiments. Data are normalized to account for the effect of miR-34a on inhibition of proliferation. A paired t-test was used to test for significant difference between means. *p < 0.05 b Glioblastoma cultures were treated as in (a) with 0–30 nM miR-34a or control and 0–100 µM TMZ in various combinations and combination index (CI) values were calculated using CompuSyn. Symbols represent actual CI data points and CI trendlines were generated by non-linear regression for A172, LN229, T98G, GBM6, GBM126, A172 TR, LN 229 TR cultures and linear regression for GBM118, LN229 TR cultures (Fa, fraction effected represents % inhibition of proliferation). CI = 1, additivity; CI > 1, antagonism; CI < 1, synergy