Fig. 5

OTULIN was required for EA to attenuate neuronal injury in focal cerebral ischaemia/reperfusion rats. a Nissl staining was used to observe the morphology of neurons in the ischaemic penumbra of the cerebral cortex. Black and red arrows represent normal neurons and injured neurons, respectively. (b, n = 3) The statistical analysis of injured neurons using Nissl staining. c Immunofluorescence was used to detect TUNEL⁺ cells and TUNEL⁺NeuN⁺ cells. Panels d (n = 3) and e (n = 3) present the quantitative analyses of the TUNEL⁺ and TUNEL⁺NeuN⁺ cells. f NeuN and NF-κB p65 were co-stained to observe the nuclear translocation of neuronal NF-κB p65 using immunofluorescence. ***P < 0.001 versus the Sham group; &&&P < 0.001, &&P < 0.01, and &P < 0.05 versus the tMCAO group; $$$P < 0.001 and $$P < 0.01 versus the tMCAO + EA group; ###P < 0.001 and ##P < 0.01 versus the tMCAO + EA + LV-Scramble group. Scale bar = 100 μm