Fig. 2

Interfering Fendrr inhibits NLRC4-mediated pyroptosis of the high glucose (HG)-hypoxia/reoxygenation (H/R) model cells. Exposing mouse microglia cell line BV-2 cells to high glucose followed by H/R, and the cells were transfected with si-Fendrr or si-NC (control). The cells were divided into the HG-control group, the HG-H/R group, the HG-H/R + si-NC group, and the HG-H/R + si-Fendrr group (n = 3/group). a The expression of lncRNA-Fendrr was detected by qRT-PCR. b NLRC4, ASC, cleaved caspase-1 and IL-1β protein levels were detected by Western blotting. c and d The levels of IL-1β and IL-18 in the cell supernatant were measured by ELISA. e TUNEL analysis was performed on the cells. Data are presented as means ± SD. P values were analyzed by Student’s t-test. **P < 0.01 and ***P < 0.001 versus the HG-control group; #P < 0.05 and ###P < 0.001 versus the HG-H/R group