Fig. 3

MiR-181c-5p bound to HMGB1 and may have a negative correlation with HMGB1 in LPS-induced microglia. a TargetScan v7.2 (http://www.targetscan.org/vert_72/) and b dual luciferase assay were used to predict and verify the binding relationship between miR-181c-5p and HMGB1, respectively. HMGB1-WT was the group with wild-type sequence of HMGB1 inserted into a luciferase reporter plasmid. HMGB1-MUT was the group with mutant sequence of HMGB1 inserted into a luciferase reporter plasmid. N = 3 for each column; ^‡‡‡P < 0.001 t test vs MC. c After induction with different concentrations of LPS (25, 50, 100, 200 ng/mL), qRT-PCR was used to detect the expressions of miR-181c-5p and HMGB1 in microglia. β-actin or U6 was used as a control. M miR-181c-5p mimic, MC miR-181c-5p mimic control, ELISA enzyme linked immunosorbent assay, qRT-PCR quantitative reverse transcription real time polymerase chain reaction