Fig. 4

The effect of miR-181c-5p on cytokines and apoptosis in microglia. a–d The contents and mRNA levels of TNF-α, IL-1β, and HMGB1 in the Control, MC, M, LPS, LPS + M, LPS + M + HMGB1 groups were determined by ELISA and qRT-PCR, respectively. N = 3 for each column; ***P < 0.001 vs Control; ^{^^}P < 0.01, ^{^^^}P < 0.001 vs MC; ^&&P < 0.01, ^&&&P < 0.001 vs M; ^###P < 0.001 vs LPS; ^§§§P < 0.001 vs LPS + M; One-way ANOVA followed by Bonferroni's post-hoc test. e QRT-PCR was used to determine the level of miR-181c-5p in each group. U6 was served as a control. N = 3 for each column; ***P < 0.001 vs Control; ^{^^}P < 0.01, ^{^^^}P < 0.001 vs MC; ^&&P < 0.01, ^&&&P < 0.001 vs M; ^###P < 0.001 vs LPS; ^§§§P < 0.001 vs LPS + M; One-way ANOVA followed by Bonferroni's post-hoc test. f The apoptosis of microglia in each group was detected by TUNEL. N = 3. Each experiment was repeated three times independently. M miR-181c-5p mimic; MC miR-181c-5p mimic control; ELISA enzyme linked immunosorbent assay; qRT-PCR quantitative reverse transcription real time polymerase chain reaction