Fig. 6

CircPTK2 partially offset the effect of miR-181c-5p on cytokines and apoptosis in microglia. a–d The contents and mRNA levels of TNF-α, IL-1β, and HMGB1 in the Control, siNC, sicirc, LPS, LPS + sicirc, LPS + sicirc + I groups were detected by ELISA and qRT-PCR. N = 3 for each column; ***P < 0.001 vs. Control; ^{^^}P < 0.01, ^{^^^}P < 0.001 vs. siNC; ^&&&P < 0.001 vs. siCirc; ^###P < 0.001 vs. LPS; ^§§§P < 0.001 vs. LPS + siCirc; One-way ANOVA followed by Bonferroni's post-hoc test. e QRT-PCR was used to determine the level of miR-181c-5p in each group. N = 3 for each column; ***P < 0.001 vs. Control; ^{^^}P < 0.01, ^{^^^}P < 0.001 vs. siNC; ^&&&P < 0.001 vs. siCirc; ^###P < 0.001 vs. LPS; ^§§P < 0.01 vs. LPS + siCirc; One-way ANOVA followed by Bonferroni's post-hoc test. f The apoptosis of microglia in each group was detected by TUNEL. Sicirc sicircPTK2; ELISA enzyme linked immunosorbent assay; qRT-PCR quantitative reverse transcription real time polymerase chain reaction. β-actin or U6 were used as control