Fig. 3
From: Redox modifications of cysteine residues regulate the cytokine activity of HMGB1
The time-dependent inhibitory effect of H2O2 exposure on the TNF-α-stimulating activity of HMGB1 in a RAW 267.7 cells and b primary human macrophages. c The effect of H2O2 exposure on the TNF-α-stimulating activity of LPS in RAW 264.7 cells. Cells were cultured in 96-well plates and stimulated with HMGB1 or LPS with or without exposure to 50 mM H2O2 for 0–120 min. Number of experiments in each panel: n = 3. One-way ANOVA followed by Tukey’s multiple comparisons test between groups. *p < 0.05 versus HMGB1