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Fig. 2 | Molecular Medicine

Fig. 2

From: LncRNA SOX2OT alleviates mesangial cell proliferation and fibrosis in diabetic nephropathy via Akt/mTOR-mediated autophagy

Fig. 2

SOX2OT overexpression promoted autophagy and relieved DN-induced renal injury in vivo. DN mice were injected with lentivirus carrying pcDNA-SOX2OT or pcDNA-vector. a Blood glucose, 24 h proteinuria, serum creatinine, blood urea nitrogen levels in DN mice after SOX2OT overexpression. b Relative kidney weight in DN mice in response to SOX2OT overexpression. c HE, Masson and LC3 staining of renal sections in DN mice with SOX2OT overexpression. IHC staining detected fibronectin and collagen IV levels. Scale bar: 50 μm. d SOX2OT mRNA expression was determined by RT-qPCR in the kidney of DN mice with SOX2OT overexpression. e Western blot analysis of LC3II/LC3I, Beclin1 and p62 in the kidney of DN mice after SOX2OT overexpression. GAPDH was used as a loading control in western blot analysis. Error bars represent the mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, One-way-ANOVA followed by Newman-Keuls post hoc test

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Molecular Medicine

ISSN: 1528-3658