Fig. 3

MiR-206 inhibited the differentiation of Treg cells by decreasing Foxp3 expression. A Expression levels of Foxp3 in CD4⁺T cells in response to miR-206 overexpression or knockdown. B, C Schematic illustration of miR-206 binding sites in Foxp3 and direct binding of miR-206 to Foxp3 by a dual luciferase reporter assay. D, E The percentage of Treg cells in CD4⁺ T cells in response to co-overexpression or co-knockdown of miR-206 and Foxp3. All experiments were repeated three times. Data are expressed as mean ± SD. **P < 0.01; ^##P < 0.01