Fig. 2

Expression of Siglec-G by B-1a cells is dramatically reduced after sepsis, but its expression is protected in CIRP^−/− mice after sepsis. WT and CIRP^−/− mice were randomly assigned to sham or CLP groups. 20 h after CLP, peritoneal lavage samples were collected for analysis. A Representative dot blots of the gating strategy of Siglec-G expression on B-1a cells of sham and CLP mice are shown. B, C Status of Siglec-G expression by B-1a cells in terms of frequency (%) and number after surgery are shown. Data are expressed as means ± SE (n = 10–14 mice/group). The groups were compared by one-way ANOVA and Student–Newman–Keuls (SNK) method (*p < 0.05 vs. WT sham and ^#p < 0.05 vs. WT CLP mice)