Fig. 4

rmCIRP induces a downregulation of Siglec-G expression by B-1a cells in vivo and in vitro. 20 h after i.p. injection of rmCIRP, PerC lavage samples were collected. Peritoneal cells were stained with CD23, B220, CD5, and Siglec-G Abs and subjected to flow cytometric detection of B-1a cells and analysis of Siglec-G expression. Status of Siglec-G expression by B-1a cells in terms of A frequency and B number after i.p. injection of rmCIRP are shown. PerC B-1a cells were stimulated with rmCIRP in vitro for 24 h. Cells were stained with Siglec-G Ab and subjected to flow cytometric analysis. C Frequency of Siglec-G expression on B-1a cells after stimulation with rmCIRP are shown. Data are expressed as means ± SE (n = 6–7 mice/group). The groups were compared by Student’s t-test (*p < 0.05 vs. vehicle treatment)